All four CD4+ T cell subsets, T-bet+ (Th1), Eomes+ (cytotoxic), FoxP3+ (Tregs), and RORγt+ (Th17), were elevated in MASH livers compared to isolated steatosis, with T-bet+ CD4+ T cells showing a statistically significant increase (Fig. 4K). This evidence concerns the gene EOMES and steatosis.