Similar trends have been previously reported for hepatic CD4+ T cells obtained from patients with MASH.16 Th1 cells were also increased in the circulation of our MASH mice, which is consistent with previously reported increases in Th1 or IFNγ-producing CD4+ T cells in the peripheral blood of patients with MASH.20,21 CD4+ regulatory T cells (Tregs), defined as FoxP3+ CD25high cells, were also significantly increased in MASH liver, which may indicate that they are being recruited to the inflamed liver as a compensatory mechanism to suppress excessive immune responses and limit tissue damage. Here, CD4 is linked to metabolic dysfunction-associated steatohepatitis.