We further show the decrease in cholinergic signaling observed in the CF model guts may be clinically relevant as restoring sensitivity to cholinergic signaling rescues many CF pathologies in Drosophila. Finally, we identify Forkhead (Fkh), a FOXA1/A2 homolog as a transcriptional regulator of Ace expression in the CF model gut. This evidence concerns the gene FOXA1 and cystic fibrosis.