To establish the presence of ICAM-1 in atherosclerotic lesions in our experimental animal model, we conducted immunofluorescence analysis (IFA) to examine the expression of ICAM-1 on luminal endothelium in the aortas of 22-week-old ApoE−/− mice fed with HFD to recapitulate atherosclerosis. This evidence concerns the gene ICAM1 and atherosclerosis.