Among negatively selected genes, 43 DDR genes—including well-characterized tumor suppressors and DDR regulators (e.g., BRCA2, CUL5, MRE11, RNF4, XRCC2, FANCA, XRCC1, SLX4, RAD54B) showed significant reductions in sgRNA abundance following PARPi treatment. Here, CUL5 is linked to neoplasm.