PTEN, a critical tumor suppressor, negatively regulates the PI3K/AKT/mTOR pathway, and its loss further exacerbates GBM aggressiveness.264 The inhibition of mTOR with rapamycin has demonstrated promising effects in vitro, but clinical trials have shown limited efficacy,265 suggesting the need for combination therapies. The gene discussed is MTOR; the disease is glioblastoma.