CDKN2B-AS1 and cancer: To explore the potential mutual regulation between 66CTG and c-Myc, we utilized siRNAs to silence the expression of c-Myc in BT549 and MDA-MB-468 cells and found that c-Myc knockdown, similar to 66CTG knockdown, significantly induced G1 phase arrest in cancer cells (Supplementary Fig. 2c–f).