We used orthogonal approaches to inhibit the ACBP/DBI system (by knockdown, knockout, antibody-mediated neutralization, or receptor mutation) and to induce HCC (orthotopic inoculation of HCC cells, hydrodynamic injection of vectors expressing MYC and beta-catenin, WD plus CCl4, and WD plus DEN) that yielded similar conclusions with respect to the pro-HCC effects of ACBP/DBI. This evidence concerns the gene CTNNB1 and hepatocellular carcinoma.