Validation by RT-qPCR confirmed the upregulation of ferroptosis driver genes as well as the downregulation of ferroptosis suppressor genes in livers from (1) WD + CCl4-treated Dbi−/− versus Dbi+/+ mice, (2) WD + CCl4-treated Gabrg2F77I/F77I versus Gabrg2WT mice, (3)WD + CCl4-treated mice, and (4) WD + DEN-treated KLH-ACBP/Dbi versus KLH-only-vaccinated mice (Figure 6A). The gene discussed is DBI; the disease is Wilson disease.