We observed that knockout of Dbi (Figures 4A–4F and S7A–S7F), homozygous Gabrg2 mutation (Figures 4G–4L and S7G–S7L), as well as immunization with KLH-ACBP/Dbi (Figures 4M–4R and S7M–S7R) mitigated the stigmata of WD + CCl4-induced liver damage (MASH, lobular inflammation, ballooning with Mallory-Denk bodies, and fibrosis), reduced liver ACBP/Dbi mRNA and protein expression as well as circulating ACBP/Dbi concentrations, and inhibited the development of HCC. Here, DBI is linked to hepatocellular carcinoma.