RPA1 and sexually transmitted disease: The assay showed to be specific to and could detect as low as 282 cells/mLwith the aid of a portableLFA reader or 1.3 × 103 cells/mL by visual inspection.Evaluation with biobanked clinical samples demonstrated that the assaycould detect moderate load of , and it does not suffer any interference from DNA of other STI agents.The assay had perfect concordance with the culture assay and strongcorrelation with qPCR Cq values, with a positive RPA-NALF result inbiobanked genomic DNA samples with Cq ≤ 23.