HLA-S and small cell lung carcinoma: We next explored the relationship between NOTCH1 and EMT by performing RNA-Seq across multiple time points in our previously described H82 (NEUROD1) SCLC cell line model, in which HLAs and antigen presentation machinery (APM) genes are upregulated by NOTCH1 intracellular domain (N1ICD) overexpression (20).