Our findings advance these reports by showing that a choline-deficient diet with low n-6 PUFA (D2 group) decreases PPAR-γ and increases NF-κB, TNF-α, IL-1β, and the M1/M2 ratio, while reducing IL-4 in NASH livers, with choline deficient diet with high n-6 PUFA (D1 group) further exacerbating these inflammatory changes. The gene discussed is IL1B; the disease is metabolic dysfunction-associated steatohepatitis.