Specifically, the combination of choline deficiency and n-6 PUFA supplementation exacerbated oxidative stress (MDA levels), inflammation (NF-κB, TNF-α, IL-1β, and IL-4 levels), and macrophage polarization (M1/M2 ratio). The gene discussed is NFKB1; the disease is hyperinsulinemic hypoglycemia, familial, 4.