The study revealed that there was a decrease in the expression of mesenchymal markers Vimentin and α‐SMA in hypospadias tissues, whereas there was an increase in epithelial markers E‐cadherin, ZO‐1, β‐catenin, and Occludin (p < 0.05) as shown in Figure 1D,E. This evidence concerns the gene TJP1 and hypospadias.