The results showed that PKNOX1 was highly expressed in BRCA(breast invasive carcinoma), CHOL(cholangiocarcinoma)), COAD(colon adenocarcinoma), ESCA(esophageal carcinoma), HNSC(head and neck squamous cell carcinoma), LIHC(liver hepatocellular carcinoma), LUAD(lung adenocarcinoma), LUSC((lung squamous cell) and STAD((stomach adenocarcinoma) (Figure 1A). Here, PKNOX1 is linked to cholangiocarcinoma.