The results revealed that the highest frequency of PKNOX1 gene mutation was in gastric adenocarcinoma (3.64%), of which 7 cases (1.59%) had “mutation” and 7 cases (1.59%) had “deep deletion.” “Mutation” was most frequently found in uterine corpus endometrial carcinoma (2.65%), whereas “structural variant,” “amplification,” “deep deletion” and “multiple alterations” were commonly found in esophageal adenocarcinoma (0.55%), acute myeloid leukemia (2.5%), gastric adenocarcinoma (1.59%), and kidney renal clear cell carcinoma (0.2%), respectively (Figure 3A). This evidence concerns the gene PKNOX1 and esophageal adenocarcinoma.