Compared with thay in the normal liver cell line THLE2, PKNOX1 was significantly upregulated the five HCC cell lines (Figure 10A), with relatively high expression in HCC-LM3 and HepG2 cells and relatively low expression in MHCC97-H and PLC/PRF/5 cells. This evidence concerns the gene PKNOX1 and hepatocellular carcinoma.