Similarly, in the wound healing assay, the migration rate of PKNOX1-knockdown cells was markedly decreased after 48 hours (Figure 11H).These findings are consistent with the results observed in HCC cells, further confirming that PKNOX1 is highly expressed in most cancers and promotes tumor growth, proliferation, migration, and invasion. Here, PKNOX1 is linked to hepatocellular carcinoma.