In addition, we also found that the methylation level of PKNOX1 was significantly negatively correlated with the expression levels of 19 tumor genes, including TGCT, STAD, LUSC, etc. We further analysed the correlation between PKNOX1 and nine methylation-related genes and found that PKNOX1 expression was more closely related to YTHDC1 and YTHDF3 expression and was significantly positively correlated in most tumors. This evidence concerns the gene YTHDC1 and neoplasm.