The results revealed that in GBMLGG, LGG, LUAD, COAD, COADREAD(Colon adenocarcinoma/Rectum adenocarcinoma Esophageal carcinoma), STES, STAD, BLCA, and ACC, the expression level of PKNOX1 was significantly positively correlated with TMB. The gene discussed is PKNOX1; the disease is bladder transitional cell carcinoma.