Minimally processed uEV samples from bladder cancer (BlCa) (n = 62), benign prostatic hyperplasia (BPH) (n = 50) and healthy (n = 30) individuals were tested in sandwich UCNP‐LFIA format, capturing uEVs with the same anti‐CD63 antibody conjugated to UCNP and immobilized on the test zone. Here, CD63 is linked to urinary bladder cancer.