Minimally processed uEV samples from bladder cancer (BlCa) (n = 62), benign prostatic hyperplasia (BPH) (n = 50) and healthy (n = 30) individuals were tested in sandwich UCNP‐LFIA format, capturing uEVs with the same anti‐CD63 antibody conjugated to UCNP and immobilized on the test zone. The gene discussed is CD63; the disease is benign prostatic hyperplasia.