After recognizing tumor cells, CIK cells enable to trigger cytotoxicity via releasing granzyme and perforin, secreting killing cytokines (IFN-γ and TNF-α), inducing tumor cells apoptosis via Fas-FasL pathway, and CD16-induced perform antibody-dependent cell-mediated cytotoxicity (ADCC) [204]. This evidence concerns the gene PRF1 and neoplasm.