Genomic analysis of the TMX-treated tertiary transplanted AML cells revealed the presence of both un-recombined (Rnpc3lox) and deleted (Rnpc3Δ) alleles, indicating that TMX treatment did not achieve 100% recombination of the Rnpc3 locus in AML cells in vivo (Fig. EV3F). This evidence concerns the gene RNPC3 and acute myeloid leukemia.