Notably, all three NXF-proteins, the nucleoporins NUP214 and NUP93, SPATA5, and SPAT5L1, were also at least 3-fold enriched in a separate pulldown of NXT2 from a second independent testis lysate (lysate 2, N = 2, Supplementary Data 3 and 4) and a pulldown from a third lysate derived from pooled testicular biopsies of three men with obstructive azoospermia (lysate 3, N = 1, Supplementary Data 5). Here, NPAS4 is linked to Obstructive azoospermia.