TP53 and cancer: Furthermore, p53 is frequently mutated in cancer cells, leading to functional alterations.48 Consequently, in addition to conducting Co-IP in a p53 wild-type cell line (HCT-116), we further investigated other p53 mutant cell lines, including HT-29 (human colorectal carcinoma) and MDA-MB-231 (human breast carcinoma) cells.