First, although we demonstrated that Huaier induces ferroptosis in tumor cells by downregulating the SLC7A11/GPX4 pathway and upregulating ferritinophagy in both in vitro and in vivo models, the exact mechanisms by which Huaier causes the downregulation of SLC7A11 and the upregulation of NCOA4 require further elucidation. The gene discussed is GPX4; the disease is neoplasm.