IFNAR1 and infection: To explore the critical regulators involved in the IFN-I signaling-mediated ubiquitination pathway during bacterial infection, we performed genome-wide RNA sequencing (RNA-seq) analysis of WT and IFNAR-deficient (Ifnar–/–) bone marrow-derived macrophages (BMDMs) following infection with A. baumannii. The expression of 127 and 401 genes was significantly greater (≥2-fold) in WT BMDMs than in Ifnar–/– BMDMs under normal and A. baumannii-infected conditions, respectively (S1, A and S1B Fig and S1 Table).