To further assess the potential of Neo1 as an IAV receptor, we infected A549 cells stably overexpressing Neo1 (LV-Neo1) with A/WSN/33 at MOI 100 and evaluated their localization at 2 and 5 minutes post-infection under non-permeabilized conditions via immunofluorescence (Figs 5 and S4). This evidence concerns the gene NEO1 and infection.