Fig 5E and 5F show that in UUO and sh-NC-injected mice, BCL-2 was decreased but BAX was increased compared to the control group; Isg20 knockdown resulted in increased BCL-2 expression and decreased Bax expression compared to the UUO and UUO + sh-NC groups, indicating reduced apoptosis. These findings suggest that Isg20 knockdown potentially ameliorates ER stress and cell death by inhibiting ribosome biogenesis in the UUO-induced renal fibrosis model. This evidence concerns the gene BCL2 and renal fibrosis.