GPT and Failure to thrive: The successful induction of this well-characterized model of liver fibrosis is confirmed by the presence of elevated liver proteins in the serum, failure to thrive (reduced weight gain in 10-week-old mice compared to controls), and histological analysis.29–31 We detected elevated serum alanine transaminase (ALT) levels in mice treated with CCl4 (with or without cfAF) compared to wild-type controls (Supplementary Figure 1A).