Considering that overexpressed CARF could impair proliferation by activating p53‐HDM2‐p21 pathway,[17, 18] qPCR analysis revealed that tumors overexpressing CARFAAexhibited more significant upregulated expression of p53 and p21WAF1, while the expression of downstream negative regulator HDM2 was more reduced compared to CARF‐expressing tumors (Figure 5H), confirming the enhanced effect of CARFAAin tumor suppression. This evidence concerns the gene TP53 and neoplasm.