To determine how heterogeneous YAP/TAZ activity impacts the tumor phenotype, a YAP/TAZ double knockout (dKO) cell line was established using the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system (Ran et al, 2013), and the phenotypic differences between YAP/TAZhigh and YAP/TAZlow cells were investigated. Here, YAP1 is linked to neoplasm.