For example, proteomic profiling of EV-associated proteins combined with advanced purification methodologies has led to the discovery of novel biomarkers, such as G protein-coupled receptor class C group 5 member C (GPRC5C) and epidermal growth factor receptor pathway substrate 8 (EPS8), both of which show diagnostic potential for early-stage PC [151]. This evidence concerns the gene EPS8 and pachyonychia congenita.