SDC1 and neoplasm: To investigate the role of SDC1, we performed siRNA-mediated silencing of the SDC1 gene in C0 MYH11 + CAF, resulting in a significant reduction in SDC1 protein expression, as shown in Fig. 12B. Next, we assessed the optimal concentration of recombinant human SDC1 protein to investigate the effect of soluble SDC1 on tumor cells.