We performed RT–PCR using primers for the exon 2 of PLEKHA1 and the 3’- untranslated regions (UTR) of TACC2 to detect the predicated PLEKHA1-TACC2 transcript in tumor tissue of ESCC 1 (Fig. 1B), followed by Sanger sequencing. Here, PLEKHA1 is linked to esophageal squamous cell carcinoma.