ROCK2-knockdown in cells significantly enhanced the sensitivity of these cells to Pemigatinib resistant, and our results also showed that while inhibition of ferroptosis with FDO significantly reduced the cell death induced by ROCK2 knockdown, none of the other inhibitors (Z-VAD, Ne, or 3-MA) had a comparable effect in CCA cells (Figs. 2R–T, S4A–G, S5A–G). This evidence concerns the gene ROCK2 and cholangiocarcinoma.