To investigate the role of E2F4 in SNRPB2-promoted proliferation, migration, and invasion of ESCC cells, we constructed overexpression plasmid of E2F4 to cotransfect with shSNRPB2 in TE-1 and KYSE-150 cells (Supplementary Figure S4e). The gene discussed is E2F4; the disease is esophageal squamous cell carcinoma.