PROM1 and hepatocellular carcinoma: Next, to better simulate the real situation in vivo, an orthotopic mouse model of HCC was constructed by intrahepatic inoculation of 2 million CD133-SK-HEP-1 cells transduced to stably express luciferase (CD133-SK-HEP-1-luc cells), and 1×107 NT, CAR-T, mCAR-T (10) or mCAR-T (100) cells were injected intravenously on days 7, 10 and 13 after inoculation (Figure 7A).