Based on the baseline expression levels of PPP1R12B across hepatocellular carcinoma cell lines (Supplementary Figure S1A), we constructed stable PPP1R12B-overexpressing cell lines in Huh7, HepG2, and MHCC-97H (with relatively low natural expression), and stable knockdown cell lines in PLC/PRF/5 and CSQT-2 (with relatively high endogenous expression). Here, PPP1R12B is linked to hepatocellular carcinoma.