Figure 5C illustrated the differentiation trajectories from various tissue origins. The differentiation trajectory of TCs was divided into seven phases, with findings indicating that the highest proportion of C3 FN1+ TCs, the earliest subtype in development, was observed in stage 1 (Figure 5D). The violin plots illustrating the developmental sequence indicated that omental tissues developed prior to ovarian cancer tissues, exhibiting a statistically significant difference, with C3 FN1+ TCs identified as the earliest subtype of TCs (Figures 5E,F). The gene discussed is FN1; the disease is ovarian cancer.