According to the findings of the investigation, the C3 FN1+ TCs subtype were found to be spatially connected with tumor sites, and their positions were shown to be consistent with the distribution of myeloid cells and circulating FN1. Through further analysis utilizing stLearn, it was shown that FN1, which was largely expressed by C3 FN1+ TCs subtype and fibroblasts, interacted with the receptor CD44 through paracrine and proximal secretion. This evidence concerns the gene C3 and neoplasm.