Immunohistochemical staining showed that the tumoroids from all the groups were positive for β‐Catenin, CDX‐2, CK20, and E‐Cadherin, and negative for MUC‐2, which were the featured markers of colorectal cancers, as shown in the Protein Atlas database (Figure 2B). This evidence concerns the gene CDX2 and colorectal cancer.