Luciferase immunostaining also showed that there was an increased LN metastasis rate in the PC3-EVcircPDLIM5 group (Fig. 4G and H).Moreover, the expression level of LYVE-1 in murine tumor tissues was strongly correlated with EVcircPDLIM5(Fig. 4I).Collectively, these results suggest that EV-mediated circPDLIM5 significantly facilitates the LN metastasis of PCa in vivo. Here, LYVE1 is linked to neoplasm.