CRISPR-mediated deletion of IRF8 (Supplementary Fig. 9B) in control human anti-Lewis Y CAR T cells did not significantly modulate cytokine production or expression of memory and exhaustion markers TCF-1, TIM-3 or PD-1 in unstimulated or tumor stimulated CAR T cells (Supplementary Figs. 9C–E). This evidence concerns the gene PDCD1 and neoplasm.