PRF1 and acute myeloid leukemia: A similarly featured antigen is TIM-3 (T-cell immunoglobulin mucin-3), which is expressed in LSCs in almost all types of AML but not in HSCs.398–400 TIM3-CAR-T cells exhibited robust antileukemia activity in xenograft models, accompanied by the production of IFN-γ, granzyme B, and perforin.224