Subsequently, western blot analyses revealed that the downregulation of MESP1 expression led to an increase in Bax protein levels, a decrease in Bcl2 protein levels, enhanced cancer cell apoptosis rate, suppression of EMT, and a reduction in metastasis of PC cells (Fig. 5A, B and Supplementary Fig. S1E, F). This evidence concerns the gene BCL2 and pachyonychia congenita.