To directly elucidate the nature of the core carbohydrate linkages (i.e., N- versus O-glycans) that display E-selectin-binding carbohydrates on CD45, we assessed the E-selectin binding capacity of CD45RABC-E after employing two well-validated techniques to trim/remove N-glycans from AML cells: enzymatic removal of N-glycans using peptide-N-glycosidase F (PNGase; New England Biolabs) and metabolic inhibition of N-glycosylation using kifunensine. The gene discussed is PTPRC; the disease is acute myeloid leukemia.