PTPRC and acute myeloid leukemia: In a complementary approach, using an E–Ig as a probe, we immunoprecipitated all E-selectin ligands from AML cells, from PBMCs, and from peripheral blood leukocytes (obtained from buffy coat of whole blood cells), followed by CD45 staining Western blot analysis to detect if E-selectin-binding isoglycoforms of CD45 were present among these cells.