To dissect whether either NCTD or eIF4G cleavage could cause inhibition of de novo nucleotide metabolism during infection, we made use of recombinantly generated CVB3–2Am + L and CVB3–2Am + Lpro, which lack the catalytic activity of 2Apro, but include either a functional L from EMCV or a functional Lpro of FMDV (Fig 4B). The gene discussed is EIF4G1; the disease is infection.