In the present study, we employed an in vitro model of MASLD, which consisted of 3D multilineage hepatic spheroids, composed of hepatic stellate LX-2 and hepatoma HepG2 cells, homozygous for the PNPLA3 I148M sequence variant, the strongest genetic determinant of MASLD. The gene discussed is PNPLA3; the disease is metabolic dysfunction-associated steatotic liver disease.