Although there were no significant differences in the aortic sinus mean plaque area among the three groups (Figure 6—figure supplement 1), detailed analysis of the aortic root plaques at five different levels revealed significantly greater lesions in Apoe-/- mice that were administered CCR4-deficient Tregs than in Apoe-/- mice that were administered CCR4-intact Tregs (Figure 6B), suggesting that the impaired CCR4-deficient Treg function is involved in the acceleration of atherosclerosis in Ccr4-/-Apoe-/- mice. Here, APOE is linked to atherosclerosis.