The results comparing the mutation load between the two groups showed that the VAF of TET2 was significantly higher in the AF group (p < 0.05), with a trend towards an increase in V617F%, whereas no significant differences were observed in the VAFs of ASXL1, DNMT3A, FAT1, and EP300 between the two groups (Figure 1A, Table S3). Here, EP300 is linked to atrial fibrillation.