The results indicated that the three vaccines enhanced the expression levels of immune-related factors of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) (p < 0.01), provided immune protection against bacterial infections (p < 0.01), effectively reduced kidney bacterial counts (p < 0.01), and increased the phagocytic activity of white blood cells in C. auratus (p < 0.01). The gene discussed is NDUFAB1; the disease is bacterial infectious disease.