Notably, Temporal GeneTerrain adeptly delineates immediate and delayed transcriptional responses to single and combination treatments with mefloquine, tamoxifen, and withaferin A. This temporal dissection reveals that pathway activations, such as NGF-stimulated transcription and the unfolded protein response, occur in a staggered manner, aligning with studies that emphasize transcriptional adaptation as a key driver of tumor progression rather than a reflection of pre-existing cellular states (Bolis et al., 2021). This evidence concerns the gene NGF and neoplasm.