Because ASD is often co-morbid with attention-deficit/hyperactivity disorder (ADHD) and anxiety disorder (Al Ghamdi and AlMusailhi, 2024; Guerrera et al., 2022; Hours et al., 2022; van Steensel et al., 2011), we next sought to determine the effects of NEXMIF overexpression on hyperactivity and anxiety-like phenotypes in NEXOX mice. This evidence concerns the gene NEXMIF and Anxiety.