Those risk mutations [espA(Rv3616c:4055801), espK(Rv3879c:4357597), espE(Rv3864:4340330), espl(Rv3876:4353557), eccE1(Rv3882c:4362807), eccC5(Rv1783:2019942), esxU(Rv3445c:3863138), esxO(Rv2346c:2626018) and esxW(Rv3620c:4060588)] were also predicted to negatively affect the respective proteins that affect the protein instability in nearby structural areas, supporting the hypothesis that they may affect TB transmission. The gene discussed is TLE4; the disease is tuberculosis.