Short-term (3 days) HFD feeding plus ethanol binge significantly aggravated liver injury and steatosis in mice as indicated by the increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and triglyceride (TG) levels, the upregulated hepatic TG levels, and Oil Red O staining and H&E staining. This evidence concerns the gene GPT and steatosis.