Confocal imaging (Fig. 4 (a) and Fig. S2) of these infections showed increased depths of invasion of wt N. gonorrhoeae, the his-tagged mutant (lig E-hiskan) and the overexpressor (opaB-lig E-hiskan) in the tissue model, while Δlig Ekan remained on the upper surface of the tissue. Here, UBE2K is linked to infection.