TNF and Anxiety: Following the intervention with probiotics, the group with FD and anxiety experienced significant decreases in IL-1, IL-6, TNF-α, LPS, Zonulin, and DAO (p < 0.001), as well as significant reductions in the Patient Assessment of Gastrointestinal Disorders-Symptom Severity Index (PAGI-SYM) and the Hamilton Anxiety Scale (HAMA) scores (p < 0.001).