Next, a protein synthesis inhibitor cycloheximide (CHX) was used to confirm the regulatory effect of USP10 on POLR2A stability, and we observed that the degradation of POLR2A was positively correlated to the reduction of USP10 expression in both USP10 depleted and overexpressed HNSCC cells (Figure 7B,C; Figure S7D,E, Supporting Information). The gene discussed is POLR2A; the disease is head and neck squamous cell carcinoma.